How the Service Works
Submit your sequence in one-letter or three-letter amino acid code, along with desired scale, purity grade and any modifications. Within one business day our chemists return a quotation that includes a free difficulty assessment: we flag aggregation-prone stretches, beta-branched clusters, acid-labile motifs and D-amino acid placements, and suggest — only where genuinely useful — strategies such as pseudoproline dipeptides, backbone protection or double coupling. Production begins after your approval, and you receive the batch record, HPLC chromatogram and mass spectrum with the final lyophilized peptide.
Synthesis Capabilities
| Parameter | Specification |
|---|---|
| Chemistry | Fmoc/tBu solid-phase peptide synthesis (SPPS), microwave-assisted for difficult sequences |
| Length | Dipeptides to 100+ residue polypeptide chains |
| Scale | 1 mg research scale to multi-gram GMP-like batches |
| Purity grades | Crude, desalted, ≥75%, ≥85%, ≥95%, ≥98%, ≥99% |
| Turnaround | 2–3 weeks standard; 5–8 business days rush available |
| Quality documentation | Batch-matched COA with analytical HPLC and MS (MALDI-TOF / ESI) |
Modification Menu
Modifications are what turn a linear chain into a research tool. We routinely deliver:
- Terminus chemistry: N-terminal acetylation, C-terminal amidation, N-terminal free amine or free acid variants
- Non-natural residues: D-amino acids, beta-amino acids, Aib, norleucine, ornithine, hydroxyproline
- Chromophores & labels: FITC, TAMRA, Alexa Fluor, biotin, DOTA, lanthanide chelators
- Phosphorylation: pSer, pThr, p Tyr for kinase substrate and SH2-domain studies
- Cyclization: disulfide bridges (single and multiple), head-to-tail cyclization, staples via olefin metathesis
- Lipidation & conjugation: palmitoylation, myristoylation, PEG spacers, KLH/BSA carrier conjugation for antibody production
- Isotope labeling: selective 15N / 13C incorporation and NMR-ready uniformly labeled peptides
- Quenched fluorescence substrates: Dabcyl/Edans FRET pairs for protease assays
Purification and Release Testing
Crude cleavage products are purified on preparative reverse-phase C18 HPLC using water/acetonitrile gradients with TFA or alternative counterions. Fractions are pooled by analytical purity, lyophilized, and re-tested before release. Net peptide content is determined by quantitative amino-acid analysis or UV absorbance where an extinction coefficient can be calculated. This distinction between chromatographic purity and net content — explained in plain language in our peptide science guide — matters when you calculate how much peptide is actually in your vial, and we report both numbers on every COA.
Difficult Sequences Welcome
Long hydrophobic stretches, highly basic sequences such as poly-Arg cell-penetrating peptides, repeat motifs and “impossible” 80-mers are routine work for our team. If another peptide company has declined your project or delivered low yield, send us the sequence: our free review costs nothing and often identifies a fixable design issue rather than a synthesis impossibility.
Request a Quote
Email your sequence and requirements to [email protected] or use our contact form. Sequences are treated as confidential by default; NDAs are available on request. See also our analytical technology overview and the peptide testing guide for how independent labs verify synthesis quality.